Dependence of the anticodon stem conformation of tRNA Trp on the anticodon loop structure

After conversion of the exposed cytosine bases of the anticodon loop into uridines with bisulfite the pattern of hydrolysis of the tRNATrp anticodon stem under the action of cobra venom endonuclease is altered. A removal of the 3'-CCA end has no influence on the hydrolytic pattern of the anticodon stem in tRNATrp. Thus, the nucleotide sequence of the anticodon loop influences the conformation of the neighbouring stem.