Influence of technique and transplantation site on rosette formation in rabbit retinal transplants

. In order to determine mechanical and host-graft related interactions in the histogenesis of retinal transplants, a new technique for transplanting flat and comparatively large pieces of embryonic rabbit retina into adult rabbit eyes was elaborated. With the procedure, free-floating grafts in the epiretinal space survive, develop and differentiate largely without rosette formation, suggesting that the dissection and transplantation procedure is adequate for obtaining a normal development. On the other hand, subretinal transplants mature at an apparently faster pace than epiretinal transplants, but do not become regularly laminated. Outer segments do not develop well in the epiretinal transplants, whereas they do so in the subretinal ones, suggesting host-graft interactions by means of yet unknown diffusible factors.