The Effects of Fuzheng Huayu Decoction on the Cell Cycle Regulation in Hepatic Precancerosis Induced by DEN in Rats

Objective To investigate the effects of Fuzheng Huayu decoction on the expression of CyclinD1 and CDK4 protein in precancerous lesions of liver cancer induced with DEN in rats, and reveal the treatment mechanism. Methods Eighty SD rats were randomly divided into four groups: blank group, model group, Fuzheng Huayu decoction group and Oxymatrine group. During the experiment, Fuzheng Huayu decoction and Oxymatrine injection were used to interfere the hepatocarcinogenesis induced by DEN in rats. After 18 wk, the expression of CyclinD1 and CDK4 protein of hepatocyte was detected with immunohistochemistry technology. Results Compared with that in the model group, pathological changes in liver tissue of Fuzheng Huayu decoction group and Oxymatrine group had obviously improved; The expression of CyclinD1 and CDK4 protein of the two treatment groups was significantly different from that in the model group (P 0.05). Between the Oxymatrine group and Fuzheng Huayu decoction group, the expression of CyclinD1 and CDK4 protein had distinctly statistical difference (P 0.05). Conclusion Fuzheng Huayu decoction can effectively prevent the precancerous lesions of liver cancer in rats, which is related to regulate expression of Cyclin D1, CDK4.