Spectrofluorometric Determination of Putrescine: Optimization of the Putrescine-Orthophthaldehyde Complex Using Spectrofluorometry.

In alkaline medium, the complex formed between putrescine and orthophthalaldehyde was studied using spectrofluorescence. The derivative is kinetically stable 24 h after complexation. The stoichiometry of the complex is 1:1 at maximum fluorescence intensity, also 24 h after complexation.