Определение антибиотиков тетрациклинового ряда в молоке методом высокоэффективной жидкостной хроматографии с послеколоночной реакцией и флуориметрическим детектированием

Conditions of sensitive and selective chromatographyic determination of tetracycline complexes with Mg 2+ in milk using HPLC with post-column reaction and fluorescence detection are specified. The fluorescence intensity of chelates is shown to be up to 1.8 times higher in microemulsions compared to that in water-acetonitrile medium. The detection limits are 5.8 and 25 ng/ml for tetracycline, oxytetracycline and doxycycline, respectively.