Randomized study using biotherapeutic and #8220;T.cruzi 3dH and #8221; impairs experimental infection by Trypanosoma cruzi

Objective: The aim of this study was to evaluate the effect of a biotherapeutic in very low dynamization (3dH) in murine infection by Trypanosoma cruzi. Methods: Swiss male mice (Mus musculus) infected by T. cruzi Y strain were divided into three groups according to their treatment: Control infection - 7% ethanolwater solution; 3dH each day (ED) - daily biotherapeutic T. cruzi 3dH, from the day of infection until the end of experiment; 3dH single day (SD) - biotherapeutic T. cruzi 3dH, on the day of infection for 12 h. Parasitological (total parasitemia, peak of parasites, day of the peaks, prepatent period, patent period, and survival) and clinical (body temperature and body weight) parameters were evaluated. Results: No significant differences were observed among groups for parasitological evaluation. 3dH ED group presented an earlier mortality compared to control. Clinical parameters showed that animals treated with biotherapeutic had worse outcome when compared with control animals. Conclusion: The low dynamization of T. cruzi biotherapeutic worsened its murine infection by means of clinical evaluation. Considering other studies using biotherapeutic of T. cruzi, suggests the possibility for an efficacy of different dynamizations regarding oscillatory potency-effect-curves.