Layer-Enriched Tissue Dissection of the Mouse Placenta in Late Gestation

Chapter Summary This protocol describes a dissection procedure for collecting layer-enriched tissue samples from the mouse placenta in late gestation. Sample enrichment for decidua, spongiotrophoblast, labyrinth, and chorionic plate is shown using histology and mRNA expression of layer-specific markers. Sample enrichment can be used to aid quantitative detection of layer-specific changes in variables such as mRNA and protein levels.