Amplification oligonucleotides met / fret labeled and method relate to its use.

In the present invention ARE PRESENTED labeled oligonucleotides amplifying nucleic acids, which may be linear or hairpins U PRIMERS blocking oligonucleotides. Oligonucleotides of the invention labeled with donor FRACTIONS AND / OR PEER acceptor energy transfer MOLECULAR. FRACTIONS CAN BE fluorophores FLUORESCENT ENERGY TO BE ISSUED BY THE DONOR be absorbed by the acceptor. The acceptor MAY BE A fluorophore that fluoresces A wavelength DIFFERENT FROM FRACTION donor, or may be a quencher. Oligonucleotides of the invention is configured so that in the amplification product REMAIN INCORPORATED AND A FRACTION a donor acceptor moiety. In the invention are also presented PROCEDURES AND SCREENING KITS FOR DIRECT PRODUCTS Amplification using primers AMPLIFICATION Nucleic Acid. WHEN USING LABELED PRIMERS LINEAR, treatment with exonuclease TEMPERATURE OR SPECIFIED eliminates the need for having to separate Primers NOT been incorporated. THIS FORM IN "closed tube" GREATLY REDUCE THE POSSIBILITY OF A DRAG WITH POLLUTION amplification products ALLOWS OBTAINING A WEALTH OF SAMPLES AND CAN BE FULLY AUTOMATE.