Comparative analysis of differentially expressed miRNAs related to uterine involution in the ovine ovary and uterus
2021
Abstract. To examine the possible miRNA molecular regulatory mechanisms
during maternal uterine involution after delivery, we selected ovary and
uterus tissues that are structurally connected as experimental materials. We
employed Illumina HiSeq sequencing to screen and analyze the quantity and
characteristics of miRNA in postpartum ewes in the methylergometrine-treated
group and physiological saline control group. Results showed that 16 miRNAs
were identified in the ovary libraries, including 4 known miRNAs and 12
novel miRNAs. In the uterus libraries, 54 miRNAs were identified, which
included 5 known miRNAs and 49 novel miRNAs. At the same time, target gene
prediction, GO annotation, and KEGG signaling pathway enrichment analysis
were employed. We found that maternal uterine involution after delivery may
involve two miRNA-target gene pairs, i.e., miRNA-200a-ZEB1 and YAP1. The
YAP1/Hippo signaling pathway is used to construct an ovary–uterine axial
regulatory mechanism to regulate the restoration of postpartum maternal
uterine morphology and function. In view of this, the identification of
miRNAs with significant differences in this study fills a gap in research on
miRNAs associated with regulation of postpartum uterine recovery in ewes and
provided an important reference for comprehensive understanding and in-depth
research on the regulatory molecular network mechanism for postpartum
uterine involution in small ruminants.
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