On Ovary and Embryo Culture of Hybrid between Rapeseeds and Rocketsalad

During the ovary culture, the ovary growth amount is the largest while the amount of embryos and seedlings is the most in the treatments of ovaries excised 6~8 days after pollination.By using the hormone culture medium of difference concentration and associations hormone,including non-hormone,to handle the material,the hybrid embryos of the Xiangyou15 and Rocketsalad can be both obtained,and the association of 1.0 mg/L NAA+0.5 mg/L 6-BA has the best effect. When two kinds of hormones are employed separately,they can enhance forming embryo amount and forming seedling amount of the Xiangyou15 and Rocketsalad. During the embryo culture,the growth hormone and cytokin have obvious accelerating effect on its development,but the effect isn抰 obvious while attached component(casein )is employed alone, however,the effect is better when it is employed with growth hormone.The ovary and embryo culture can both attain some forming seedling amount,but through the identification of genuine and sham hybrid seedling stage, 24 genuine hybrid strain all come from the ovary culture.