Mining New Bt Insecticidal Protein Genes Resource Using Illumina Sequencing Technology

Bacillus thuringiensis ( Bt ), as the most widely used biologically produced pest control agent, is already auseful alternative or supplement to synthetic chemical pesticide and is also a key source of genes for transgenic expression to provide pest resistance in plants. On basis of Bt strains isolated from soil samples from the Diaoluoshan and Wuzhishan Natural Reserve, we carried out a large-scale screening the insecticidal genes from the special Bt strains through Illumina sequencing. In this study, 25 of the insecticidal genes, including 5 of the novel vegetative insecticidal protein genes ( vip ) and 3 of the new cyt genes had been identified from the different Bt strains. All the result indicated that the second generation sequencing technology of illumine is efficient in mining the insecticidal gene from Bt isolates, which would further enrich the new different insecticide genes application in commercial agriculture, forest management, and mosquito control.