Содержание и ДНК-связывающая активность ядерного транскрипционного фактора NF-kB в опухолях больных раком молочной железы

Concentration and DNA-binding activity of nuclear transcription factor NF-κΒ p65 subunit were measured by quantitative immunoenzymatic methods in the tumors and histologically unchanged mammary gland tissues of 119 breast cancer patients. Enhanced as compared to adjacent tissue NF-kBp65 DNA-binding activity was revealed in 97% of the tumors, and in most cases it was associated with an increase of total NF-kBp65 content. No significant associations were found between the parameters studied and disease stage, tumor size and histology, lymph node status, but NF-kBp65 content was significantly increased in grade III as comparedto grade II tumors. HER2 + tumors were characterized by an increase of total NF-kBp65 expression independent on steroid receptor status and not accompanied by enhancement of DNA-binding activity, the latter being the highest in “triple receptor negative” (ER -PR -HER2 -) tumors.