N-terminus methylation of Desulfovibrio vulgaris (miyazaki) F cytochrome c3

Cytochrome c 3 from Desulfovibrio vulgaris (Miyazaki) F is an electron-transfer protein between hydrogenase and ferredoxin and other types of cytochromes. In this study, an N-terminus of the cytochrome c 3 has been identified as an N-methylated alanine residue by cleavage with peptidase, liquid chromatography/mass spectrometry (LC/MS) and nuclear magnetic resonance (NMR) analysis. Heterologous expression of the cytochrome c 3 with Shewanella oneidensis TSP-C is not methylated, this indicate that the N-methylation occur in vivo in D. vulgaris.