Prokaryotic Expression and Protein Purification of Soybean GmGBP1 Gene

A new SKIP homolog in soybean was constructed into prokaryotic expression vector pGEX-6p-1 and then transformed into E.coli BL21(DE3)to get the expression protein for further study.The result indicated that an 95 kDa recombinant protein was expressed with the treatment of 0.1 mmol·L-1IPTG for 8 h at 37℃,the recombinant protein was confirmed to mainly existed in inclusion body form by SDS-PAGE.The high-quality recombinant protein was obtained through GST column purification after the inclusion body was dissolved with 8 mol·L-1 urea.