Study on Pretreatment of Improving Corn Stalk Enzymatic Hydrolysis Yield

【Objective】Three different pretreatment methods were tested to study the effect on hydrolysis of cellulose from corn stalk.【Method】The dilute-acid,dilute-alkali and sodium sulfite anhydrous pretreatment of corn stalk were carried out,followed by cellulose enzymatic hydrolysis.【Result】The results showed that the optimum condition for enzymatic hydrolysis is at temperature of 50℃,pH of 4.8,ratio of solid to liquid 1∶30,enzyme concentration of 2.7 g/L,and the hydrolysis time of 24 h.Under the optimized condition,the rate of enzymatic hydrolysis on corn stalk pretreatment using sodium sulfite anhydrous was 39.07%,which is four multiple higher than that of corn stalk hydrolysis without pretreatment.【Conclusion】Pretreatment destroyed the fibrous structure of corn stalk significantly.Relative good rate of enzymatic hydrolysis on corn stalk can be achieved by sodium sulfite anhydrous pretreatment.