The Characterization of Catecholamine Stimulation of Glucose Transport by Rat Adipocytes and Isolated Plasma Membranes

These studies were designed to resolve the controversy over the ability of catecholamines to stimulate glucose transport per se and not just glucose utilization in adipocytes. Glucose transport assays were performed using a dual label (3- O-[3H]methylglucose and l-[14C]glucose) procedure. Fifteenminute preincubations of intact adipocytes with epinephrine produced a 15% increase in specific 3-O-methylglucose transport at 5 × 10−8 m, while a maximal stimulation of twice the control level was reached at 2.5 × 10−7 m. This stimulation of glucose transport was inhibited 95% by 10−5 m propranolol. Isoproterenol (10−7 m) also stimulated 3-O-methylglucose transport, and this effect was completely blocked by 10−5 m propranolol. Phenylephrine at maximal doses (5 × 10−6 m) produced a marginal (20–30%) stimulation of transport which was only partially (50%) inhibited by phentolamine. Epinephrine and insulin stimulation of 3-O-methylglucose transport were compared, and the maximal stimulation of transport by epinephri...