Biochemical enzyme activity in different tissues of rats exposed to a novel phosphorothionate (RPR-V).

The effects of a novel phosphorothionate (RPR‐V) synthesized at Indian Institute of Chemical Technology, Hyderabad, was studied using three sub‐chronic doses of 0.033 (low), 0.066 (medium) and 0.099 (high) mg kg−1 in male and female rats for a period of 90 days. This long term and repeated treatment of RPR‐V revealed that the membrane bound target enzymes Aspartate aminotransferase and Alanine aminotransferase increased significantly in serum and kidney, whereas these enzymes significantly decreased in liver and lung tissues when measured after 45 and 90 days of treatment. This compound also caused significant inhibition of RBC Acetylcholinesterase, target enzyme of organophosphorus compounds revealing its effect on normal synaptic transmission. Two way Anova studies disclosed that the alterations were mostly dose and time dependent, sexual dimorphism was not observed when the activities of male rats were compared with female rats. Enzyme recoveries were recorded after 28 days of post treatment, high degr...