Determination of EPA,DPA and DHA in Seal Oil by Gas Chromatography

Objective: To establish a method for determining ω-3 polyunsaturated fatty acids including eicosapentaenoic acid(EPA),docosapentenoic acid(DPA) and docosahexaenoic acid(DHA) in seal oil.Methods: Fatty acids in seal oil were methyl esterified using a KOH-methanol solution.The applicability of the developed method was validated.Results: This method revealed an excellent liner relationship for methylated EPA,DPA and DHA in the concentration range of 10-2000μg/mL with recovery rate of 96.93%-103.38% and RSD less than 2%.Conclusion: This method is simple,rapid,accurate and suitable for the analysis of fatty acids in seal oil.