Inferring the spatial code of cell-cell interactions and communication across a whole animal body

Cell-cell interactions are crucial for multicellular organisms as they shape cellular function and ultimately organismal phenotype. However, the spatial code embedded in the molecular interactions that drive and sustain spatial organization, and in the organization that in turns drives intercellular interactions across a living animal remains to be elucidated. Here we use the expression of ligand-receptor pairs obtained from a whole-body single-cell transcriptome of Caenorhabditis elegans larvae to compute the potential for intercellular interactions through a Bray-Curtis-like metric. Leveraging a 3D atlas of C. elegans cells, we implement a genetic algorithm to select the ligand-receptor pairs most informative of the spatial organization of cells. Validating the strategy, the selected ligand-receptor pairs are involved in known cell-migration and morphogenesis processes and we confirm a negative correlation between cell-cell distances and interactions. Thus, our computational framework helps identify cell-cell interactions and their relationship with intercellular distances, and decipher molecular bases encoding spatial information in a whole animal. Furthermore, it can also be used to elucidate associations with any other intercellular phenotype and applied to other multicellular organisms. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=136 SRC="FIGDIR/small/392217v1_ufig1.gif" ALT="Figure 1"> View larger version (36K): org.highwire.dtl.DTLVardef@8583e9org.highwire.dtl.DTLVardef@19746adorg.highwire.dtl.DTLVardef@a11708org.highwire.dtl.DTLVardef@1ef2a3d_HPS_FORMAT_FIGEXP M_FIG C_FIG