Effect of eugenol fumigation treatment on chilling injury and CBF gene expression in eggplant fruit during cold storage

Abstract The effect of eugenol (EUG) on chilling injury (CI) to eggplant fruit ( Solanum melongena L.) was investigated. Eggplant fruit were pre-treated with 25 μL/L EUG, and then stored at 4 °C for 12 days. Results showed that EUG fumigation treatment effectively retarded the CI development, reduced pulp browning, weight loss, and malondialdehyde (MDA) content, and sustained soluble solids content (SSC) and proline content. Moreover, the activities of polyphenol oxidase (PPO) and peroxidase (POD) were inhibited by EUG. C-repeat/dehydration-responsive element binding factors (CBF) genes are transcription factors playing a critical role in cold acclimation. To illuminate the molecular regulation of EUG on chilling tolerance in eggplant fruit, a 1151 bp SmCBF gene was identified and the effect of EUG on SmCBF expression was determined by RT-qPCR. EUG resulted in a higher SmCBF expression. These findings suggested that EUG treatment had potential effect on alleviating CI in eggplant fruit.