Study on clinical value of HBV large envelope protein detected by up-converting phoshor immune chromatography assay
2013
Objective To evaluate the application value of the up-converting Phoshor technology immunochromatography for HBV large envelope protein (HBV-LP) quantitative determination strip in hepatitis B patients.Methods Serum HBV-LP was detected by a new UPT-based immunochromatograhpic technology,and HBV DNA was quantitively detected by real time fluorescent quantitation polymerase chain reaction (RT-PCR),HBV five serum markers were detected by chemiluminescence method.Results In 500 cases of patients with hepatitis B,HBV-LP and HBV DNA positive rates were 58.0% and 42.2% respectively,there was significant difference between the positive rate of HBV DNA and that of HBeAg(P < 0.01); In 215 cases of HBeAg negative specimens,the positive rates of HBV DNA and HBV-LP were 29.3% and 37.2% respectively,the difference was statistically significant (P > 0.05); and HBeAg positive rate was 57.0%,there was significant difference between the positive rate of HBV DNA and that of HBeAg (P < 0.01).Conclusion HBV-LP detected by UPT method can be used for the evaluation of viral replication and prognosis of patients with HBeAg negative and HBV DNA low copies patients.Combing detection of HBV DNA,HBV-LP and HBeAg is conducive to the judgment of HBV replication level and determination of antiviral treatment end point.
Key words:
Viral envelope proteins; Immunochemistry; Virus replication; Up-converting phosphor technology
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