New Nanoaggregates of Crosslinked Laccase for Reactive Red Bioremediation

: Environmental concerns have led to an increased interest in developing green technologies for bioremediation of wastewater pollutants. In view of this, laccases have emerged as attractive green catalysts due to their applicability in oxidizing broad range of substrates. In the present work laccase was extracted from Coriolus versicolor (MTCC 138) and stabilized by formation of new crosslinked laccase nanoaggregates (CLNAs) using two different crosslinkers-N,N-methylenebisacrylamide (N, N-MBAAm) and ethyleneglycol dimethacrylate (EGDMA). Evaluation of laccase activity profile of the free as well as the well characterized CLNAs was carried out, and these were used in reactive red (RR) bioremediation. The CLNAs showed enriched catalytic activity at different temperatures and pH than the free laccase, good reusability when studied up to six cycles, and thermal and storage stability. CLNAs exhibited good bioremediation ability, which was enhanced in the presence of 1-hydroxybenzo triazole (HBT), a laccase mediator.