A comparative biochemical study of ultrasound and interferential therapy for bone healing in rabbits

In present study 18 New Zealand White rabbit were randomly divided into 3 equal groups. For comparing the therapeutic efficacy we developed a rabbit model with 2mm bone defect in mid-shaft of right radius. The animals in the group A did not receive any physiotherapy and kept as control, whereas, groups B and C animals were treated with low intensity pulsed ultrasound (0.5 watt/cm²) and bipolar interferential therapy (100 KHz), respectively, for 10 min on alternate days up to 2 weeks. In all the animals initially there was decrease in serum calcium level up to 7th to 15th day followed by an increase. However, Group B animals exhibited significantly low calcium at 15 days as compared to other groups of animal. In animals of groups B and C, phosphorus level significantly increased from 15th day to last of the observation period, whereas, in group A animals showed these significant increased level on 30th and 60th day. In group B, also exhibited significant higher alkaline phosphatase levels and low lipid per oxidation level as compared to interferential and control group animal. In group B animals cortisol level did not vary considerably at different time intervals. However in group c animal’s significantly higher cortisol level was observed. Thus ultrasound therapy resulted in faster bone healing and early resolution of physiological stress due to creation of bone defect in comparison to interferential therapy.