Translation of third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tumor tissue

Third and second harmonic generation (THG/SHG) and two-photon autofluorescence (2PEF) microscopy is a novel imaging technique that has been suggested as a promising clinical tool, mainly for cancer diagnosis. Here, we test a compact, portable ex-vivo THG/SHG/2PEF microscope (Femto Diagnostics B.V.) in the hospital. We will present the first results of an ongoing study in which we image lung tumor tissue immediately after excision, compare the images to the golden standard histopathology, assess the quality of diagnosis by independent pathologists, and examine the clinical impact of the THG/SHG/2PEF microscope. We also focus on identification and distinction of different immune cells.