Procjena utjecaja onečišćenja tla kadmijem i živom na sastav dušika u listu miskantusa primjenom VNIR spektroskopije
2020
In this paper, the influence of soil contamination with heavy metals (cadmium and mercury)
on nitrogen content in Miscanthus x giganteus leaf, was determined. The paper is based on
estimates of biomass quantity and nitrogen content in leaf tissue using remote sensing that
make up non-destructive methods such as proximal VNIR spectroscopy. It serves as an
additional tool with analytical methods that integrate physiological and optical properties,
and as a result the state of plant tissue is obtained, i.e. the influence of heavy metals or
other stressors on the concentration of plant pigments and nitrogen content. The aim of the
study was to determine effects of cadmium and mercury on miscanthus using proximal
spectroscopy in the visible and near-infrared (VNIR) region of the electromagnetic spectrum
by monitoring nitrogen content in the leaf and aboveground biomass yield as one of the
main parameters. The effect of cadmium and mercury differed depending on the treatments
of different soil improvers to which miscanthus seedlings were exposed in experimental pots
inside an open greenhouse. Leaf reflection was measured on 24 samples at the end of the
2018./2019. and 2019./2020. vegetation seasons with a transmission spectroradiometer in
the wavelength range of 350─1050 nm. The yield of aboveground biomass was determined
by weighing, while the content of total nitrogen in the leaf was determined by the method of
dry incineration. Partial least squares regression (PLSR) was used to display and process the
results, which determined a strong correlation for the aboveground biomass yield (R2 = 0.53;
RMSE = 0.833 t/ha), and a complete correlation was found for the composition of the total
nitrogen content in the leaf (R2 = 0,87; RMSE = 0,139%). In terms of treatment, sewage
sludge showed the greatest contribution in the total nitrogen content in the aboveground
biomass of miscanthus, while the ash proved to be the least useful improver in the first year
of cultivation, and soil without amandments and mycorrhiza in the second year of cultivation.
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