Method for extracting DNA from salmon can

2016 
The invention discloses a method for extracting DNA from a salmon can .Further, the method is used for identifying the salmon variety in the salmon can .The method includes the following steps that firstly, a certain mass of salmon can sample is weighed, and DNA is extracted through a DNA extraction solution, wherein the DNA extraction solution is prepared from 4% CTAB, 100 mmol/L Tris-HCl (pH 8.0), 1.4 mol/L NaCl and 20 mmol/L EDTA (pH 8.0); secondly, the DNA obtained in the first step serves as a template, and 212 bp DNA segments of COI are amplified; thirdly, the segments amplified in the second step are sequenced, sequence features are compared in an existing DNA database, and salmon variety information is obtained .The method for successfully extracting the DNA used for identifying the salmon variety in the salmon is built, and has very important practical significance in improving the quality and safety level of can industrial products in China, standardizing industrial development and protecting legal rights and the right to know of customers.
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