Process for preparing viable bacillus licheniformis preparation

2014 
The invention relates to a process for preparing a viable bacillus licheniformis preparation. According to the process, after bacillus licheniformis undergoes first-stage germ amplification culture and second-stage germ amplification culture, the bacillus licheniformis is inoculated to a fermentation culture medium for culture, and the number and conversion ratio of bacilli is increased through feeding a supplement carbon source during fermentation, so that the unit yield is increased remarkably, and the unit cost is reduced; at the end of fermentation, fermentation liquor is concentrated with a vacuum film concentrator, so that soluble synergistic substances, growth promoting substances and bacteriostatic active substances can be effectively recovered from the fermentation liquor; the concentrated fermentation liquor is subjected to drying and granulating by multi-level low-temperature spray-fluidized drying equipment, granules are subjected to sorting, fine powder gathered by a cyclone separator returns and is granulated again, and then, a bacillus licheniformis product, of which the CFU of powder per gram is not less than 2.0*10 , is obtained; exhaust gas is treated and is discharged after reaching standard. The process has the advantages that effective substances in the fermentation liquor can be reserved to the maximum, the quality of the product is high, the equipment utilization ratio is high, the retention of biological activity is relatively good, waste gas and waste water are discharged after reaching standard, and large-scale industrial production can be achieved.
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