An updated genotype classification system for Zika viruses

Despite the rapid spread of the Zika virus throughout the Americas in 2015, its phylogenetic classification is limited to either an African or Asian genotype. This classification no longer reflects the present genetic diversity of circulating strains and their geographic reach. Using 414 publicly available Zika virus genomes from 40 different countries, we created alignments of different genomic fragments with high phylogenetic signal and used these to construct multiple maximum likelihood trees (IQTree). We observed groups of strains from three major geographic regions that consistently cluster into monophyletic clades: African, Asian and American, the latter of which cluster into four further sub-clades: Caribbean, South American (SA1 and SA2) and Central American. The inter-genetic distances of these clades are all significantly greater than their intra-genetic distances (p=0.05). A decision demonstrates that only five nucleotide positions are needed to correctly classify 90% of our dataset into the newly described genotypes.