Extended-Spectrum Properties of CMY-30, a Val211Gly Mutant of CMY-2 Cephalosporinase

CMY-30, a Val211Gly mutant of CMY-2 cephalosporinase, was derived by mutagenesis. The hydrolytic efficiency of CMY-30 against expanded-spectrum cephalosporins was higher than that of CMY-2 due to increased kcat values. Findings indicate a role of the Ω loop residue 211 in determining the substrate specificities of CMYs also corroborated by modeling studies.