Cytotoxic effect of niobium phosphate glass-based gutta-percha points on periodontal ligament fibroblasts in vitro
2020
INTRODUCTION The outcome of root canal obturation might be affected by the chemical components of the chosen filling materials. Niobium phosphate glass-based gutta-percha (GNB) was proposed as a biomaterial-based obturation point. This study aimed to investigate the cytotoxic and cell modulation effects of GNB points on human periodontal ligament fibroblasts (PDLF) in vitro METHODS: Human PDLF were cultured for the assays. Extracts of regular gutta-percha points (GP) and GNB were obtained, serially diluted (1:5, 1:10, and 1:25), and used to stimulate PDLF. Cell viability assay was performed using Alamar blue reagent, and RT-qPCR was used to assess the gene expression for collagen type 1 (COL-1) and cementum protein 1 (CEMP-1). A one-way analysis of variance followed by Tukey's post-test was performed (p < 0.05) RESULTS: Regular GP reduced cell viability only in pure extracts, while GNB exhibited cytotoxicity to PDLF in pure extracts as well as 1/5 and 1/10 dilutions. The gene expression of COL-1 was downregulated only in the GNB group (p < 0.05). The expression of CEMP-1 remained unaltered by both tested materials. CONCLUSION The addition of niobium phosphate glass to GP points increased cytotoxicity, affecting PDLF viability and partially disturbing physiological cell function.
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