Rapid Identification of Coagulase Negative Staphylococcus Isolates from Mastitic Milk by RFLP Analysis of PCR-Amplified groEL Gene (HSP60)

The aim of this study was to evaluate of reliability the technique of restriction fragment length polymorphism (RFLP-PCR) of groELgene for Coagulase Negative Staphlococcus (CNS) of animal origin. For this reason, 47 CNS species were identified by the API Staph testkit. Genotypic characterization, based on RFLP-PCR of the groEL gene, was performed on 20 CNS isolates. The most commonly identifiedCNS species were Staphylococcus epidermidis 21(44,7%) and Staphylococcus haemolyticus 11 (23,4%) followed by Staphylococcus xylosus7 (14,9%). Acording to PCR-RFLP profiles, 5 different species were identified: Staphylococcus cohnii, Staphylococcus epidermidis,Staphylococcus haemolyticus, Staphylococcus hyicus and Staphylococcus xylosus. In conclusion, PCR-RFLP of the groEL gene is apotentially precius assay for accurate molecular identification of CNS species. Most of the commercial identification sistems are not disignedto identify veterinary pathogen. This method must be developed for CNS of animal origin.