Mapping of fertility-restoring gene for wild-abortive type cytoplasmic male sterility in a japonica rice restorer line SWR78.

According to the fertility segregation of F2 and BC1F1 populations derived from a cross between Suqiu A with wild-abortive type cytoplasmic male sterility(CMS) and SWR78(a japonical rice restorer line),it could be presumed that the fertility restoration of this cross was controlled by three genes.Then,individuals with absolutely abortive pollen of F2 population were used to map the fertility-restoring gene.The Rf4 gene was mapped on the long arm of chromosome 10 flanked by the markers RM5629,RM5373,STS10-17 and STS10-18 at the distances of 0.17,0.03,0.03 and 0.07 cM,respectively.The Rf4 gene was localized between RM5373 and STS10-17,and the physical distance of the interval between the two markers was about 78 kb.