Human α2-macroglobulin as an inhibitor of insoluble trypsin

Abstract α 2 -Macroglobulin binds to insoluble trypsin bound on agarose beads inducing a reduction of proteolytic activity of the enzyme towards large substrates such as azocasein. When trypsin was bound on other matrices like sheep red blood cells or latex beads, the inhibition of proteolytic activity by α 2 -macroglobulin was complete. These results show that α 2 -macroglobulin inhibits similarly both soluble and insoluble proteinases.