Isolation of peanut lectin by affinity chromatography on polyacrylamide-entrapped guar beads and polyacrylamide(co-allyl α-d-galactopyranoside)☆

Abstract Guar gum (galactomannan) was entrapped in polyacrylamide gel by bead polymerization. The gel beads were used in one-step affinity column chromatography of peanut lectin (specific for d -galactosyl residue). The lectin prepared with these gel beads was homogeneous by electrophoresis, either in the presence or absence of sodium dodecyl sulfate. Another affinity gel prepared by copolymerization of allyl α- d -galactopyranoside and acrylamide was also useful in purification of the lectin.