Research on Preparation of Carrier with Carbomer Gel as Substrate for Melanocytes Transplantation by New Spreading Method

Objective To prepare a carrier with carbomer gel as substrate for melanocytes transplantation by spreading method.Methods Normal human melanocytes were cultured in vitro.Then,a semi solid medium with carbomer gel was prepared as substrate.The toxicity and proliferation of the melanocytes were tested by Trypan blue and 3-(4,5-Dimethyl-2-Thiazolyl)-2,5-Diphenyl Tetrazolium Bromide(MTT).Transwell culture plates were employed to assay the migration of melanocytes.Results 1% carbomer gel had no negative impact on the activities and proliferations of melanocvtes(P0.05).The carbomer gel didn't inhibit the migration of melanocyte after 12 h(P0.05).Conclusion The mixture of carbomer gel does not injure the biological function of melanocytes,which is suit for preparing the carrier for melanocyte transplantation.