Two-Dimensional Infrared Correlation Spectroscopy

2D-IR spectroscopy can be of great help in extracting more information from the spectra. 2D-IR correlation maps obtained from artificial bands with known changes in band parameters give rise to characteristic patterns. Biological systems of varied complexity will give rise to 2D-IR correlation maps that reflect the changes induced by the perturbation and can be interpreted as a composite of the band simulation maps. The maps can be studied along the full perturbation process correlating changes in the overall pattern with changes in the properties of the protein, e.g. enzyme activity or intrinsic fluorescence, or the perturbation process can be dissected in several steps to detect early changes in the perturbation process.