Stimulated Raman Spectroscopic Imaging by Microsecond Delay-Line Tuning

We report microsecond-scale acquisition of simulated Raman spectra by resonant delay-line tuning. Our scheme improved the spectral acquisition speed by 100 times compared to previous works by motorized translational-stage tuning. 4-D images ( $\mathrm{x}-\mathrm{y}-\mathrm{z}-\lambda$ ) from highly dynamic organelles in live C. elegans was demonstrated.