Fourteen selective media facilitate evaluation of populations of coexisting fixed bacterial strains of enteric pathogens and normal human fecal flora

Abstract Selective media for evaluation of populations of fourteen fixed bacterial strains were prepared. The species used were Salmonella typhimurium, Vibrio parahaemolyticus, Yersinia enterocolitica, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Staphylococcus aureus, Enterococcus faecalis, Bacteroides fragilis, Fusobacterium varium, Clostridium perfringens, Clostridium ramosum, Clostridium difficile and Bifidobacterium adolescentis . All selective media except for Y. enterocolitica promoted growth of only the target bacteria. On the selective agar plates, 50% or more of the viable cells of the target bacteria formed colonies. In contrast, bacteria other than the target one did not grow even when inoculated with 10 7 cells per plate.