Preparation of cucumber seed peptide-calcium chelate by liquid state fermentation and its characterization

Abstract A specific cucumber seed peptide (CSP) was successfully produced by liquid state fermentation with B. subtilis HYT. Then the CSP was divided into four kind molecular weight components (CSP, untreated; CSP1, MW > 10 kDa; CSP2, 6 kDa  COOH, OH, NH 2 as well CO NH might participate in the coordination with Ca 2+ to form the CSP3-Ca chelate. Meanwhile, the finding showed that cucumber seeds peptide could chelate with calcium and play the role of novel carries for calcium supplement.