Development of an 8 miniSTR and Amelogenin fluorescent-multiplex system

Objective To develop a fluorescent multiplex system of 8 non-CODIS miniSTR and Amelogenin for forensic purpose.Methods Eight highly polymorphic non-CODIS miniSTR loci,D20S1082,D6S474,D12ATA63,D9S1122,D2S1776,D1S1627,D3S4529 and D2S441 were selected,and a fluorescent multiplex system including these loci and the genderspecific Amelogenin was developed through redesigning primers,labeling fluorescence dye,and optimizing experiment conditions.204 randomly selected individuals of Guangzhou Han population,members of 30 families and 30 degraded samples were genotyped using this system.Results Tested by the new multiplex system,all the loci can generate stable and full profiles with amplicons less than 200bp in size,which resulted in an increased overall typing success rate for degraded DNA samples.The cumulative power of discrimination and probability of exclusion were 0.99999993 and 0.992287 respectively in Guangzhou Han population.Conclusion The established fluorescent multiplex system was robust,sensitive and stable,and is of high value in forensic application.It can serve as a new approach for the analysis of degraded DNA as well as an effective supplementary of commercial kits.