Effect of Sevoflurane and Desflurane on Erythrocyte Deformability during Ischaemia-Reperfusion Injury of Lower Extremity in Diabetic Rats

Aim: It is known that blood viscosity and erythrocyte aggregation are increased and erythrocyte deformability is decreased in diabetic patients. Ischemia reperfusion injury (I/R) in lower extremity is a frequent and important clinical phenomenon. Blood rheology is known to be affected by numerous factor including anaesthetic drugs. Accordingly, we aimed to investigate the effects of sevoflurane and desflurane on erythrocyte deformability in infrarenal aorta of diabetic rats undergoing I/R Materials and methods: In this study, 30 male Wistar albino rats. After the effects of chronic diabetes encountered diabetic rats were randomly assigned into diabetic control (group DC), diabetic I/R group (group D-I/R), diabetic I/R group with desflurane (group D-I/R-D), and diabetic I/R group with sevoflurane (group D-I/R-S) groups. Another 6 rats without diabetes were assigned as control group (group C). 4 weeks after the injection of streptozotocin diabetic rats were anaesthetized by desflurane 6% or sevoflurane 2% at a dose by which minimal alveolar concentration (MAC) for rats would be one. The drugs were given for 4 hours within 100% oxygen at a rate of 4L.min 1 . Erythrocyte samples were obtained from heparinized whole blood samples. Measurements for deformability were conducted on erythrocyte suspensions within serum physiologic tamponized with phosphate. Results: Deformability index was significantly increased in diabetic rats (p<0.001), however, it was similar in GroupD-I/R, GroupDI/R-D and GroupD-I/R-S (p=0.570, p=0.951 respectively). It was significantly increased in GroupD-I/R,D-I/R-D and D-I/R-S when compared to GroupDC (p=0.001, p=0.004, p=0.001 respectively). Relative resistance was increased in diabeticand I/R models. Conclusion: Neither sevoflurane nor desflurane caused a negative effect on erythrocyte deformability in infrarenal aorta of diabetic rats undergoing I/R diabetic rats. However these findings should be further investigated in larger and more detailed studies.