Elimination of antibiotic resistance genes in waste activated sludge by persulfate treatment during the process of sludge dewatering

Abstract Two sludge conditioning modes (nanoscale zero valent iron modified by Ginkgo biloba L. leaf (G-nZVI)/sodium persulfate (PS) conditioning with different ratios (1:0, 1:0.1, 1:1 and 1:10) and G-nZVI/PS conditioning with continuous addition) in reducing the specific resistance of filtration (SRF) and removing antibiotic resistant genes (ARGs) were investigated. After 3 min, the SRF values decreased in following order: 2.45 × 108 m/kg (1:10) > 5.95 × 106 m/kg (1:0.1) > 3.72 × 106 m/kg (1:0) > 4.92 × 105 m/kg (1:1). In the continuous addition (1:1), the SRF value decreased from 1.04 × 108 m/kg to 6.47 × 106 m/kg at 9 min. Removal efficiency of ARGs was 2–6 orders of magnitude and no regeneration of ARGs was observed in sludge and water phase. When treated samples were incubated for 36 h, dominant microflora was negatively correlated with ARGs. This study revealed persulfate treatment could achieve dewatering and remove ARGs, simultaneously.