Compared with metherd of preparation Pb antigen

The glutaraldehyde and diazotization reactions were utilized to couple the bifunctional chelating agent [(R)-2-Amino-3-(4-Aminophenyl)Propyl]-Trans-(S,S)-Cyclohexane-1,2-Diamine-Pentaacetic Acid(P-Nh2-Bn-Chx-A"-Dtpa) and BSA to obtain the protein chelating complex,which was further chelated with lead ion,forming two lead holoantigens BSA-P-Nh2-Bn-Chx-A"-Dtpa-Pb with the lead antigen binding protein ratio 1:15.64 and 1:42.95.On account of the relatively low coupling rate of glutaraldehyde method,the research focused on the optimization of it.The protein chelating complex and lead ion were chelated through two steps to get the glutaraldehyde antigen BSA-P-Nh2-Bn-Chx-A"-Dtpa-Pb with up to over 1:20 coupling rate.This lead antigen's immunogenicity prepared by the above three methods had been inspected in rats immune experiments,in which antigen BSA-P-Nh2-Bn-Chx-A"-Dtpa-Pbthe prepared by two-stage chelating method presented best immunogenicity in mice with over 1:102400 immunized potency.