Pelobium manganitolerans gen. nov., sp. nov., isolated from sludge of a manganese mine.
2016
A Gram-staining-negative, rod-shaped, motile by gliding and facultative anaerobic bacterial strain, YS-25T, was isolated from a sludge of a manganese mine. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YS-25T formed a single branch within the strains of the family
Sphingobacteriaceae
and showed low similarities to
Pedobacter arcticus
CCTCC AB 2010223T (91.7 %), ‘
Pedobacter zeaxanthinifaciens
’ TDMA-5 (91.5 %),
Pedobacter terricola
DS-45T (90.9 %),
Pedobacter ureilyticus
THG-T11T (90.9 %),
Pseudopedobacter beijingensis
MCCC 1A01299T (90.8 %) and
Pedobacter heparinus
CCTCC AB 209030T (88.5 %). Strain YS-25T had some unique physiological and biochemical characteristics: facultative anaerobic, able to hydrolyse Tween 40, positive for cystine arylamidase and negative for mannose assimilation and β-galactosidase. The polar lipids of strain YS-25T were phosphatidylethanolamine, aminolipid, two unknown lipids and two glycolipids. The presence of glycolipids and absence of sphingolipid were different from the
Pedobacter
and
Pseudopedobacter
strains. The major fatty acids (>5 %) were iso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1
ω7c), unknown ECL 13.565, iso-C17 : 0 3-OH, iso-C17 : 1
ω9c and anteiso-C15 : 0. The genomic DNA G+C content was 42.0 mol%, and the only respiratory quinone was menaquinone 7. On the basis of polyphasic taxonomic analyses, strain YS-25T is considered to represent a novel genus and species, for which the name Pelobium manganitolerans gen. nov., sp. nov. is proposed. The type strain is YS-25T (=KCTC 52203T=CCTCC AB 2016051T).
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