Complete Genome Sequence of Geobacillus sp. Strain GHH01, a Thermophilic Lipase-Secreting Bacterium.

sp. GHH01 was deter-mined by a combined approach of 454 GS-FLX Titanium XLpaired-end sequencing (454 Life Sciences, Branford, CT) and Ge-nome Analyzer II single-read sequencing (TruSeq Chemistry, Il-lumina, San Diego, CA), resulting in average coverages of 10.91-fold and 33.03-fold, respectively. The assembly employing theMIRA v3.4.1.1 software (2) yielded 84 contigs 3 kbp. Gap clo-sure and quality improvement were performed by PCR-basedtechniques and subsequent Sanger sequencing (ABI 3730xl, LifeTechnologies, Carlsbad, CA). Initial gene prediction was per-formed with IMG/ER (3), followed by manual curation based oncomparisons to the Swiss-Prot, TrEMBL (4), and InterPro (5)databases. For the identification of rRNA and tRNA genes,RNAmmer v1.2 and tRNAscan-SE v1.4 (6, 7) were used, respec-tively.The complete genome consists of a 3,582,992-bp chromosomewith a G C content of 52.3%. In total, 3,597 genes were identi-fied, including 10 rRNA gene clusters and 88 tRNA genes. Theannotation resulted in 2,724 protein-encoding genes with as-signed functions.16S rRNA gene phylogenetic analysis confirmed the affiliationof