Photoacoustic Spectroscopy Applied to the Study of

We used Photoacoustic Spectroscopy (PAS) to study protoporphyrin IX (PpIX) in mice skin induced from δ- aminolevulinic acid (ALA) administrated by intraperitoneal route to CD 1 female mice. From obtained PAS spectra and by using the Phase-Resolved Method we estimated that PpIX in skin was preferentially concentrated near to the inner side of the basal membrane. It was also obtained the total attenuation coefficient (μ t) of the mouse skin as a function of wavelength. The obtained μτ values, in particular at 630 nm agree with the literature values for similar samples. Also it was estimated in a first approximation the degradation time of PpIX when is irradiated at its maximun optical absorption wavelength