Improved methods of retroviral vector transduction and production for gene therapy.

ABSTRACT To facilitate clinical applications of retroviral-mediated human gene transfer, retroviral vectors must be of high titer and free of detectable replication-competent retroviruses. The purpose of this study was to optimize methods of retroviral vector production and transduction. Studies were conducted using 22 retroviral vector producer cell lines. Inactivation of retroviral vectors was greater at 37°C than at 32°C. A 5- to 15-fold increase of vectors was produced at 32°C compared to 37°C; the vector increase at 34°C was intermediate. For example, PA317/G1Na.40 grew to a titer of 1.8 × 107 cfu/ml at 32°C, compared to 5.0 × 105 cfu/ml at 37°C. The production of retroviral vectors was scalable achieving similar results in flasks, roller bottles, or a CellCube Bioreactor. Retroviral vectors were concentrated 15–24 times with vector recovery ranging from 91 to 96% in a Pellicon tangential flow filtration system. Retroviral supernatants were successfully lyophilized. The combination of glucose or sorb...