The First Isolation and Identification of Campylobacter jejuni from Miniature Pigs in China

Objective To isolate and identify Campylobacter jejuni in miniature pig in China.Methods Specimens of a diseased Guizhou miniature pig were collected.Bacteria isolation and culture,biochemical identification,susceptibility test,serum agglutination test,PCR assay were conducted.Results One strain of Campylobacter jejuni (CJp0812) was isolated and identified.The genome of CJp0812 strain could be amplified by Campylobacter jejuni fla A genes-specific primer in PCR assay.Through determination of the nucleotide sequence,the highest sequence homology of this strain of bacteria could be demonstrated with Campylobacter jejuni NCTC11168(NC002163) and it was found to be 99%.Conclusion It is concluded that the strain CJp0812 appears to be Campylobacter jejuni first isolated from miniature pig in China,and it provides reliable science data for further research and epidemiological investigation.