Extended One-Generation Reproductive Toxicity Test in Rats Exposed to 3-Nitro-1,2,4 Triazol-5-One (NTO), October 2013-March 2014

Abstract : Rats were given ad libitum access to NTO in drinking water at four concentrations (0, 144, 720, or 3600 mg/1 NTO). Treatment of the P generation began two (females) to four (males) weeks pre-mating and continued until weaning of litters. direct dosing of F1 animals occurred from waning through puberty. Number and sex of pups, stillbirths, live births, and the presence of gross anomalies were determined on post-natal day (PND) 0/1. Ano-genital distance (AGD) was measured on PND 4 and males were examined for the presence of nipples on PND 13. F1 offspring were examined daily for vaginal opening (VO) and preputial separation (PPS). At termination, blood samples were collected for clinical chemistry, hematology, and thyroid hormone analyses and a gross necropsy was conducted. Mating index, fertility index, pre-coital interval, gestation index, litter size, number of live and stillborn pups, and sex ration did not differ among control and NTO treated groups. Nipple retention was increased in NTO exposed males compared to controls. Age at PPS was increased by 2.6 days in the 3600 mg/l group compared to controls.