A rapid and highly selective paper-based device for high-throughput detection of cysteine with red fluorescence emission and a large Stokes shift

A paper-based device is a promising technology for point-of-care testing (POCT). In this work, a novel fluorescent probe with red emission (650 nm) and a large Stokes shift (∼170 nm) for Cys detection was reported for the first time. This probe exhibited a highly selective and rapid response to Cys within 5 min. Importantly, by introducing this probe into microfluidic paper-based analytical devices (μPADs), a paper-based device was developed for simple, high-throughput and highly sensitive detection of Cys. Finally, satisfactory performance of this paper-based device for Cys detection was achieved in real samples, which indicates the great potential of our strategy in the construction of POCT systems for detecting analytes.